(100) HDL berreraikien erabilera plasma lipoproteinen dinamikak aztertzeko

Laburpena

Gaixotasun kardiobaskularrak dira heriotza-kausa nagusiena gaur egun, eta HDL partikulek kolesterolaren kanporaketan funtsezko rola dute. HDL partikulen imitatzeko sortutako rHDL nanopartikulak terapia potentzial gisa aztertzen ari dira. Lan honetan, DPPCz osatutako rHDL partikulak sintetizatu eta giza plasmarekin inkubatu dira, proteina eta lipidoen eskuratzea aztertzeko. Fluoreszentzia-markaketa eta kromatografia sekuentzialaren bidez isolatu dira, eta proteomikako emaitzek erakutsi dute HDL naturalean ohikoak diren apolipoproteinak bereganatzen dituztela. Honek adierazten du rHDLek HDL partikulak imitatzeko ahalmena dutela eta terapia kardiobaskular berrietarako bidea zabal dezaketela. Cardiovascular diseases are currently the leading cause of death, and HDL particles play a fundamental role in cholesterol efflux. Reconstituted HDL (rHDL) nanoparticles, designed to mimic HDL particles, are being investigated as potential therapeutic agents. In this study, DPPC-based rHDL particles were synthesized and incubated with human plasma to analyze their acquisition of proteins and lipids. They were isolated using fluorescent labelling and sequential chromatography, and proteomic results showed that they acquire apolipoproteins commonly found in natural HDL. This indicates that rHDLs have the capacity to mimic HDL particles and could open new avenues for cardiovascular therapies.